Product Info Summary
SKU: | A03826 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-DDX6 Antibody
SKU/Catalog Number
A03826
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DDX6 Antibody catalog # A03826. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03826)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human NBAS protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03826 is reactive to DDX6 in Human, Mouse, Rat
Applications
A03826 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.417kDa
Background of DDX6
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Mark H. L. Lambermon, Mol. Cell. Biol., Jun 2002; 22: 4346 - 4357.
Kyoung-Seok Ryu, J. Biol. Chem., Sep 2003; 278: 36621 - 36627.
R. Jrgen Dohmen, J. Biol. Chem., Jul 1995; 270: 18099.
Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
IHC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of DDX6 using Anti-DDX6 Antibody (A03826).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDX6 antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-DDX6 Antibody A03826
Western blot analysis of DDX6 on different lysates using anti-DDX6 antibody at 1/500 dilution.Positive control:
Lane 1: Human colon
Lane 2: SH-SY-5Y
Lane 3: SiHa
Lane 4: K562
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For DDX6 (Source: Uniprot.org, NCBI)
Gene Name
DDX6
Full Name
Probable ATP-dependent RNA helicase DDX6
Weight
54.417kDa
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase p54; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD box protein 6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); EC 3.6.1; EC 3.6.4.13; FLJ36338; HLR2DEAD box-6; Oncogene RCK; probable ATP-dependent RNA helicase DDX6; RCKP54 DDX6 HLR2, IDDILF, P54, RCK, Rck/p54 DEAD-box helicase 6 probable ATP-dependent RNA helicase DDX6|ATP-dependent RNA helicase p54|DEAD (Asp-Glu-Ala-Asp) box helicase 6|DEAD (Asp-Glu-Ala-Asp) box polypeptide 6|DEAD box protein 6|DEAD box-6|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)|oncogene RCK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX6, check out the DDX6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX6 Antibody (A03826)
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No publications found for A03826
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Customer Reviews
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